Extended Data Fig. 5: Validation of organelle fractionation and antibodies.
From: Regulation of liver subcellular architecture controls metabolic homeostasis
a, Immunoblot analysis of the indicated proteins and validation of the organelle fractionation. TL: total liver lysate, ER: endoplasmic reticulum, Nuc: nucleus, Mito: mitochondria, Cyto: cytosolic fraction. b, Immunoblot analysis and quantification (c) of indicated proteins in rough and smooth ER fractions from livers of lean (n = 3) and obese (n = 3) mice. The quantification of all the proteins in these blots were normalized to signal for Calnexin shown in Extended Data Fig. 4c (*p < 0.038, **p < 0.0085), as these samples were from the same experiment. d, Immunoblot analysis for Reticulon 4A (Rtn4a) and 4B (Rtn4b) in total lysates from Hepa1–6 cells expressing GFP control or Rtn4A tagged with GFP or transfected with shRNA control (scrambled, Scr) or shRNA against Reticulon4. e, Immunoblot analysis for Climp-63 in total lysates from Hepa1–6 cells expressing GFP control or Climp-63 tagged with RFP or Myc or transfected with shRNA control (scrambled, Scr) or shRNA against Climp-63. f, Immunoblot analysis for RRBP1 (p180) in total liver lysates derived from wildtype (Wt) and RRBP1 deficient mice. All data (Extended Data Fig. 5c) are presented as mean values +/− SEM. Unpaired t-test was used for all the statistical analyses.
