Fig. 1: Cryo-EM structures of different conformational states of Ste2.
From: Activation mechanism of the class D fungal GPCR dimer Ste2
a–d, Cryo-EM density map (left) and structure model (right) for Ste2–Ant (a), Ste2IL–Ag (b), Ste2AL–Ag (c) and ligand-free Ste2 (d) (Ste2A (blue), Ste2B (green), CHS molecules (purple), NAG (N-acetyl glucosamine) molecules (black)). Ligands are coloured according to the protomer to which they are bound (red or yellow). e–h, The orthosteric binding pocket of Ste2A (rainbow) showing ligands bound to Ste2–Ant (e), Ste2IL–Ag (f), Ste2AL–Ag (g) and Ste2–Ag–G (h). Densities were visualized using UCSF ChimeraX36 and encompass a carve radius of 2 Å. The contour levels used for the maps were 0.0163 (a), 0.386 (b), 0.659 (c), 0.6 (d), 0.022 (e), 0.282 (f), 0.7 (g) and 0.03 (h). h is reproduced from ref. 1.
