Extended Data Fig. 1: Purification and structure determination of antagonist-bound, ligand-free and agonist-bound Ste2.
From: Activation mechanism of the class D fungal GPCR dimer Ste2
a-c, Representative size-exclusion chromatography traces and SDS-PAGE analysis of purified agonist-bound, antagonist-bound and ligand-free Ste2, respectively. The peak fraction (red arrows) was used for SDS-PAGE analysis. Material eluting in the void volume comprised of aggregates (blue arrow). On the SDS-PAGE gels, lanes contain molecular weight markers (M) and purified Ste2 (P). Experiments were repeated three times for agonist-bound Ste2, twice for antagonist-bound and twice for ligand-free Ste2 with similar results. d–g, 2D class averages of the cryo-EM data for antagonist-bound, ligand-free, agonist-bound inactive-like and agonist-bound active-like Ste2, respectively.
