Extended Data Fig. 8: Cryo-EM data processing strategy.
From: Differential assembly diversifies GABAA receptor structures and signalling
a, A typical cryo-EM data processing workflow. The numbers in this case are derived from the α4β3δ "apo" (+HEPES) dataset. b, Soft mask used to separate receptors with 2 or 3 Nb25 bound, which largely (but not completely) separates α4β3δ and β3δ receptors. The separation is incomplete in all cases because Nb25 may also bind weakly to the β3+/α4− interface. c, To further separate α4β3δ and β3δ receptors, a soft mask around the putative position of the vestibule glycan on the α4 subunit and the putative position of the N149 glycan on the β3 subunit is used. d, Soft mask around the α4 vestibule glycan used to separate α4β3γ2 from β3γ2 receptor subtypes.
