Extended Data Fig. 1: Validation of Blood Vessel Enrichment (BVE) protocol. | Nature

Extended Data Fig. 1: Validation of Blood Vessel Enrichment (BVE) protocol.

From: Single-cell dissection of the human brain vasculature

Extended Data Fig. 1

a. qPCR of canonical cell type markers for endothelial Cldn5 (p < 0.0001), Abcb1a (p = 0.0002), Mfsd2a (p = 0.9556), mural Pdgfrb (p = 0.0388), Acta2 (p < 0.0001), Myh11 (p < 0.0001), astrocytes Aqp4 (p >0.9999), Aldh1l1 (p >0.9999), oligodendrocytes Mog (p > 0.9999), neurons Rbfox3 (p >0.9999), and microglia Aif1 (p > 0.9999) from mouse cortex, ordinary one-way ANOVA, *p < 0.05, **p < 0.01, ***p < 0.001,****p < 0.0001, n.s. = not significant. Error bars denote standard deviation of the mean from n = 3 independent biological replicates. b. Representative immunofluorescence of blood vessels enriched from mouse cortex using the BVE protocol. n = 3 independent biological replicates for immunostaining. Brightness and contrast enhanced for visualization. Scale bar, 20 μm.

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