Extended Data Fig. 3: Integrative analysis of ex vivo, post mortem, and mouse datasets.

a. UMAP plot of integrated human snRNA-seq datasets without covariate correction shown by platform and cell type and b. with covariate correction by platform and cell type c. Cell fraction distribution of single nuclei across all datasets by cerebrovasculature cell type. d. Cell number distribution of single nuclei across all datasets by cerebrovasculature cell type. e. Venn diagram overlap of genes between human post mortem vs. mouse and human ex vivo vs. mouse. f. Cell fraction and g. gene comparison of vascular cell types between mouse and human datasets. h–i. Representative functional enriched terms of human- and mouse-specific/highly expressed genes in endothelial (h) and pericytes (i). Human-mouse differentially expressed genes (hmDEGs) smooth muscle cells (j, left), and fibroblast (k, left). X-axis represents the log-transformed fold change and y-axis represents the maximal expression level. The top genes are highlighted in blue for mouse and red for human. Genes that were also cell type markers are bolded. j–k. (right panels), the representative functional enriched terms of human- and mouse-specific/highly expressed genes.