Extended Data Fig. 8: Ribosome profiling revealed that global readthrough is largely restricted to UAG in the liver.
From: AAV-delivered suppressor tRNA overcomes a nonsense mutation in mice
a, Metagene plot showing normalized reads of ribosome protected fragments (RPFs) relative to the distance from the normal stop codon at position 0. RPFs from untreated (blue, n = 3 mice), or rAAV9.2xsup-tRNATyr-treated KI/KI male mice (red, n = 3 mice) are overlaid. b, Magnified view of the 3′UTR showing increased RPFs in this region in the rAAV9.2xsup-tRNATyr treated mouse livers. c, Box plot of ribosome readthrough score (RRTS) derived from ribosome profiling of KI/KI mouse livers with (+) or without (-) rAAV9.2xsup-tRNATyr treatment (n = 3 per group). RRTS values were calculated for transcripts harboring different normal stop codons, namely UAA (orange), UAG (blue), and UGA (green), respectively. Center line indicates the median, the box ends indicate the first and third quartiles, and the whiskers indicate the range of the remaining data excluding outliers. d, Scatter plot showing RPF densities at each codon (codon occupancy) in KI/KI mouse livers with (+) or without (-) rAAV9.2xsup-tRNATyr treatment (n = 3 per group). Two tyrosine (Y) codons are highlighted in red.
