Extended Data Fig. 9: mRNA-seq analysis on human fibroblasts.
From: AAV-delivered suppressor tRNA overcomes a nonsense mutation in mice
Cells were infected with Lenti.sup-tRNATyr.EGFP or Lenti.EGFP as control for seven days as described in Fig. 2a. a, Volcano plot showing differentially expressed transcripts in Lenti.sup-tRNATyr.EGFP-treated cells compared to control cells. Red and blue dots denote significantly upregulated and downregulated transcripts (adjusted p < 0.01 and fold change > 2), respectively. b, Venn diagram showing the upregulated transcripts in a and published set of 1,000 human putative NMD substrates.
