Extended Data Fig. 9: Expression pattern of markers of distal airway cells in fetal and postnatal human airways.
From: Human distal lung maps and lineage hierarchies reveal a bipotent progenitor
a. UMAP shows expression pattern of indicated genes in fetal lung epithelial scRNA-seq data from Miller et al. b. Schematic representation of human fetal airway and distal tip bud. c. Immunostaining for SCGB3A2 (green) and SFTPB (grey) on 19-20 weeks old human fetal lung. Scale bar 100 µm. Insets show magnified regions. d. Immunostaining for SCGB3A2 (green), TP63 (red) and SFTPB (grey) in human fetal extrapulmonary airway. Scale bar: 100 µm. e. Representative RNA-FISH images showing the expression of SCGB3A2 (green), LGR5 (red) and PDGFRA (grey). Scale bar: 50 µm. DAPI stains nuclei (blue). f. Schematic representation of human postnatal lung showing TRBs and distal alveoli. g. Co-staining for SCGB3A2 (green) and SFTPB (grey) in 7-month-old postnatal human lung. Scale bar 100 µm. Insets show magnified regions. h. Immunostaining for SCGB1A1 (blue), SCGB3A2 (grey) and SFTPB (green) expression in postnatal human lung. Scale bar: 100 µm. i. Co-staining for SCGB3A2 (green), TP63 (red) and SFTPB (grey) expression in 7–12 months old postnatal lung. Scale bar: 100 µm. j–m. Representative immunostaining images from 7 months old postnatal human lung show the expression of (j) KRT5 (green), TP63 (red) and SFTPB (grey). Scale bars: 20 µm (top), 10 µm (bottom left) and 5 µm (bottom right). (k) SFTPB (green), FOXJ1 (red) and SCGB3A2 (grey). Scale bar: 10 µm. (l) SCGB3A2 (green) and SFTPB (red). Scale bar 10 µm. (m) SCGB3A2 (green) and SFTPC (red). Scale bar 20 µm. DAPI stains nuclei (blue). c, e–m: Insets indicate individual colour channels of regions marked in white boxes.
