Extended Data Fig. 10: Computational prediction of AT2 cellular transition dynamics.
From: Human distal lung maps and lineage hierarchies reveal a bipotent progenitor
a. PAGA graph of AT0, AT1, Immature AT1, AT2 and TRB-SC cells from healthy adult human lung. b. Cellular trajectory on AT0, AT1, Immature AT1, AT2 and TRB-SC cells as inferred by scVelo and PAGA algorithms. c. Cellular trajectory (left) and cells ordered along pseudotime (right) as inferred by Monocle 3 algorithm on of AT0, AT1, Immature AT1, AT2 and TRB-SC cells. d. Overall energy landscape view of AT0, AT1, Immature AT1, AT2 and TRB-SC cells as predicted by scEpath algorithm. e. MuTrans analysis of AT0, AT1, Immature AT1, AT2 and TRB-SC cells. Plots showing (from left to right) entropy, SFTPC level and SCGB3A2 level. f. Heat map showing expression of genes along trajectory from AT2 to AT1 predicted by Slingshot algorithm. Genes enriched in AT0s are highlighted by the black outline. g. Line plots showing smoothened expression (Loess smoothened) of select genes along Slingshot predicted AT2 to AT1 trajectory. Grey indicates 95% confidence interval derived from the smoothened fit. h. Line plots showing smoothened expression (Loess smoothened) of select genes along Slingshot predicted AT2 to TRB-SC trajectory. Grey indicates 95% confidence interval derived from the smoothened fit.
