Extended Data Fig. 3: Molecularly distinct cell types and zones in human airways.
From: Human distal lung maps and lineage hierarchies reveal a bipotent progenitor
a. Immunostaining for MUC5B (blue), MUC5AC (red), SCGB1A1 (green) and SCGB3A2 (grey) (bottom panel). Lines illustrate described zones. Scale bar: 100 µm. b. Quantification of distal airway epithelial cell types across the airway zones. Data are presented as mean ± SEM. (n = 4 for zone-1 and n = 3 for zones-2, 3 and 4). c. Co-staining for SCGB3A2 (green), SCGB1A1 (red), SFTPB (grey) in different regions of the human airway. Scale bar: 100 µm. d. Staining for SCGB1A1 (blue), MUC5B (green), SFTPB (red) and SCGB3A2 (grey) in different regions of the airway. Scale bar: 100 µm (main panel) and 20 µm (inset). e. Box plot showing the difference in cell populations identified in this study (red bar) and Habermann et al., (turquoise bar). Line in the box: median; box edges: 25/75 percentile; whiskers: 1.5 times interquartile range; points: outliers. f. Dot plot shows expression of indicated genes in TRB-epithelial cells (this study) but not in Deprez et al., scRNA-seq data from airway epithelial cells (annotated as distal airway). In panels c and d, images were stitched to show large area. Insets show zoomed images of regions highlighted in boxes. g. UMAP plots show the expression of indicated genes in epithelial cells. h. UMAP plots show the expression of indicated genes in epithelial cells. i. (top) Immunostaining for SCGB3A2 (green), SCGB1A1 (grey) and NAPSA (red) showing the expression of NAPSA in TRB-SC and TB-SC. Scale bar: 10 and 20 µm. (bottom) RNA-FISH for SCGB3A2 (green), TMEM45A (red) and SCGB1A1 (grey) showing the expression of TMEM45A in TRB-SC and TB-SC. blue (DAPI) indicates nuclei. Scale bar: 20 µm. j. PLISH for SCGB3A2 (green), SFTPB (red) and KDR (grey). Scale bar 50 µm. k. UMAPs show the expression of indicated genes in epithelial cell types. l. Bar plot showing the pathways enriched in TRB-SCs.
