Extended Data Fig. 6: Rho regeneration in the presence of excess all-trans-retinal.
From: Capturing a rhodopsin receptor signalling cascade across a native membrane
a, Schematic process of ROS opsin regenerated into rho in 10-fold excess of all-trans-retinal under continuous illumination. b, The intensity ratio of rho to opsin increases until it reaches a plateau at 3.8 min (red). Comparatively, rho remains constant in the presence of exogenous all-trans-retinal in the dark (blue). c, Zero-charge spectra of rho and opsin with 10-fold excessive all-trans-retinal present before and following 3.8 min illumination. This experiment was repeated twice.
