Extended Data Fig. 8: Monitoring the lipids that are released with rho while signalling through Gt over the illumination period from dark to light.
From: Capturing a rhodopsin receptor signalling cascade across a native membrane
a, PC lipids released as rho and opsin are ejected from disc membranes during signalling through Gt. Unsaturated lipids are designated as PC 34:1, 38:6 and 40:8 (orange) their more saturated counterparts 34:0, 38:4 and 40:6 (blue/purple). A decrease in the saturated lipids (blue and purple) relative to unsaturated is observed at the early stages of illumination (< 18 s). Lipids were detected in the positive ion mode. b, The intensities of PC 38:6 and 40:8 were manually selected to be compared with PC 38:4 and PC 40:6. Plotted is the ratio of unsaturated over saturated lipids as a function of illumination time. After 6 s and 18 s of illumination the extent of unsaturation increases. Data are presented as mean values +/− SE (n = 3).
