Extended Data Fig. 10: Gt activation across native membranes during illumination of a control and in the presence of 1 and 6.
From: Capturing a rhodopsin receptor signalling cascade across a native membrane
The ratio of GDP bound Gt to intermediate Gt represents a measure of the activation of transducin. Upon light absorption, rho activated G protein releases GDP a, at 6 s b, and after 15 s of the reaction. c, In the presence of 1 or 6 G protein signalling was accelerated and commenced even under dim red-light conditions when compared to the control experiment. Two different control experiments were performed in the absence of compounds (left hand column) but with the same concentration of DMSO used to dissolve 1 and 6. d, The second control in the presence of the same concentration of DMSO, and 1 and 6, but in the absence of rho* establish that the agonists do not compete with the nucleotide (GDP) and that signalling occurs through rho* in the native membrane. Two replicate experiments were performed for a–c and three replicate experiments were performed for d.
