Extended Data Fig. 2: Imaged hippocampal neurons are spatially selective and collectively span the available environment.

a, 14 simultaneously recorded ROIs during a single free-foraging session from one bat. Red dots represent estimated spiking events superimposed on the flight trajectories (grey; Methods). The size of each dot indicates the relative size of the deconvolved spiking event per imaging frame (per 33 ms bin; Methods). b, c, Firing density is not increased around the virtual boundary and the spatial activity recorded across neurons spans the available environment. b, Example spatial firing density for one animal. The virtual boundary that bats had to cross in order to rebait reward feeders is indicted by a dashed line. c, Spatial firing density of deconvolved neural activity (that is, ‘spikes’) for all animals (n = 7). Reward locations are indicated. Images in b, c are displayed from a top-down (XY) perspective. There was no significant difference in firing density within 1m of this boundary versus other locations in the room (P > 0.5 for all bats, two-tailed Wilcoxon Rank Sum).