Extended Data Fig. 6: Spatial analysis of EHT gene expression in CS15 human embryo.
From: Mapping human haematopoietic stem cells from haemogenic endothelium to birth
(a) tSNE plot documenting the main cell types in CS14-15 (4.5-5 weeks) AGM tissues (top, n = 3 biologically independent samples). Feature plots displaying the expression of arterial (GJA5), pre-HE (IL33, ALDH1A1), HE (ALDH1A1, KCNK17), HSC (KCNK17 and SPINK2) and liver SPINK2 progenitor (SPINK2, IL7R) markers in CS14-15 AGM samples (bottom). (b) First row, H&E staining of seven transverse sections, featuring dorsal aorta. Red arrows indicate intra-aortic haematopoietic cluster (IAHC) and green arrows red blood cells. Spatial sequencing plots showing the expression of arterial (GJA5), pre-HE (IL33, ALDH1A1), HE (ALDH1A1, KCNK17), HSC (KCNK17 and SPINK2) and liver SPINK2 progenitor (SPINK2, IL7R) markers. The default colour scale from Loupe browser was applied, which represents the log2 expression from 0 to the maximum value in the spots. Each dot is 55 μm and shows combined expression of 1–10 cells. White bars = 250μm, black bars = 1mm. (c) Immunofluorescence staining of CS15c (5 weeks) aorta for IL33, ALDH1A1, CD31/PECAM and DAPI (Section #251), CXCR4, KCNK17, CD31/PECAM and DAPI (section #254) and SPINK2, PTPRC/CD45, CD31/PECAM and DAPI (Section) #239. White bars = 200μm, black bar = 20μm. Individual antibody staining was performed minimum three times in independent embryos with comparable staining pattern.
