Extended Data Fig. 4: Documentation of cell types and programs involved in EHT.
From: Mapping human haematopoietic stem cells from haemogenic endothelium to birth
(a) UMAP plot showing contribution of each AGM sample (CS14-15, 4.5-5 weeks) (n = 3 biologically independent samples) to haemato-vascular clusters. (b) Dot plot showing HOXA expression in AGM haematovascular cells. (c) Pseudotime analysis of 833 cells from clusters 0–5 (endothelium and HSC). (d,e) Pseudotime trajectory plots showing the progression of pseudotime variable (d) and contribution of each AGM sample to trajectory (e). (f) Feature plots displaying the expression of markers of the different stages of EHT in pseudotime trajectory plot. (g) Heat map displaying unsupervised clustering of 11514 genes whose expression significantly changes over pseudotime, divided into 10 gene groups. (h) Summary table of enriched GO categories from each gene group (Fisher’s exact test). (i-k) Dot plots showing endothelial and haematopoietic lineage genes (i), cell cycle and metabolism-related genes (j) and signalling pathway associated genes (k) identified from pseudotime analysis, in clusters 0–8 and HE. HE is highlighted in blue.
