Extended Data Fig. 7: Western Blot quantification of relative abundances of mutant receptors evaluated in G13 GTPγS assays.
From: The tethered peptide activation mechanism of adhesion GPCRs
a, Relative abundances of CTF-only truncated GPR56 receptors in membrane homogenates determined by immunoblotting for anti-His tag. b, Relative abundances of CTF-only truncated LPHN3 receptors in membrane homogenates determined by immunoblotting for anti-His tag. c, Relative abundances of holoreceptor GPR56 NTFs and CTFs before and after treatment of membrane homogenates with ice-cold 6M urea. CTF was immunoblotted for via a GPR56-specific CTF antibody, and NTF was immunoblotted for via a GPR56-specific NTF antibody. *Multiple glycosylated NTF bands. Data represent the mean band intensity of western blots performed in triplicate with error bars representing +/- S.D. Unpaired, two-tailed student’s t tests were used to determine significance between wild type and mutant receptors with reduced abundances. * = p < 0.05
