Extended Data Fig. 4: Single-particle cryo-EM processing workflow and reconstructions of the GPR56/miniG13 complex.
From: The tethered peptide activation mechanism of adhesion GPCRs
a, Workflow of cryo-EM data processing for the active-state tethered agonist bound 7TM-GPR56/miniG13 complex. b, Angular distribution heat map of particle projections for 7TM-GPR56/miniG13 reconstruction. c, Gold standard Fourier shell correlation (FSC) curve for receptor and miniG13 reconstructions. Dashed line represents the overall nominal resolution of each reconstruction at 0.143 FSC calculated by CryoSPARC. d, Overall composite cryo-EM map for the 7TM-GPR56/miniG13 complex with chain assignments for its components. e, Cryo-EM density for TMs 1–7, the α5 helix of miniGα13/i and the bound tethered agonist for the 7TM-GPR56/miniG13 complex.
