Fig. 1: Overall structures of G protein-bound ADGRD1 and ADGRF1.
From: Structural basis of tethered agonism of the adhesion GPCRs ADGRD1 and ADGRF1
a, Cryo-EM maps of the ADGRD1–miniGs, ADGRF1–miniGs, ADGRF1–miniGi1 and ADGRF1(H565A/T567A)–miniGi1 complexes, coloured according to chains. The stalk and TMD of ADGRD1 are coloured orange and green, respectively; the stalk and TMD of ADGRF1 are coloured magenta and blue, respectively; the lipid LPC bound to ADGRF1 is coloured yellow; and Gαs, Gαi1, Gβ, Gγ and Nb35 are coloured cyan, gold, grey, pink and light gold, respectively. b, Structure of the ADGRD1–miniGs complex. The structure is shown in cartoon representation. The binding cavities for the stalk and G protein are highlighted by two dashed boxes and are shown in detail on the left. c, Structure of the ADGRF1–miniGs complex. The lipid LPC bound to the receptor intracellular region is shown as yellow sticks. The binding cavities for the stalk and G protein are highlighted by two dashed boxes and are shown in detail on the right.
