Fig. 3: Signalling cascade of ADGRD1 and ADGRF1.
From: Structural basis of tethered agonism of the adhesion GPCRs ADGRD1 and ADGRF1
a, Overall view of the interaction cores that are important for receptor activation. The three key interaction cores (cores 1–3) are highlighted by blue, green and magenta dashed boxes, respectively. The stalk residues FS3, LS6 and MS7 are shown as spheres and coloured orange; and the TMD residues in interaction cores 1, 2 and 3 are shown as spheres and coloured blue, green and magenta, respectively. b, d, f, Interactions within cores 1 (b), 2 (d) and 3 (f) in ADGRD1. The residues involved in interactions are shown as green sticks. Polar interactions are shown as red dashed lines. c, e, g, Interactions within cores 1 (c), 2 (e) and 3 (g) in ADGRF1. The residues involved in interactions are shown as blue sticks. h, i, Basal activity of wild-type (WT) and mutant versions of ADGRD1 (h) and ADGRF1 (i),measured by cAMP accumulation assay. Data are presented as a percentage of wild-type activity and are shown as mean ± s.e.m. (bars) from at least five independent experiments performed in technical triplicate with individual data points shown (dots). ***P < 0.0001 by one-way analysis of variance followed by Dunnett’s post-test compared to the response of wild type. #Low surface expression level (less than 40% of wild-type expression level). Extended Data Table 2 provides detailed independent experiment numbers (n), P values and expression level.
