Extended Data Fig. 6: Comparison of human TEN–TRAP–TPP1 and Tetrahymena TEN–TRAP–p50.

a, Comparison of telomerase-bound TPP1 and p50 (gray) (PDB: 7LMA). b, Superposition of human TEN–TRAP–TPP1 (colored as in Fig. 2) and Tetrahymena TEN–TRAP–p50 (gray). c, d, Overall comparison of human TEN–TRAP–TPP1 (c) and Tetrahymena TEN–TRAP–p50 (d) with the residues locate on the interfaces colored yellow. e, f, Open book views of the continuous interfaces between human TPP1–TERT (e) and Tetrahymena p50–TERT (f) shown as surface, respectively. g, h, A three-way junction shared by human TEN–TRAP–TPP1 (g) and Tetrahymena TEN–TRAP–p50¹⁰ (h). i, j, Density maps of TEN and TRAP without (i) and with (j) TPP1, highlighting the changes in density upon TPP1 binding. Density thresholds were adjusted for comparable density in the RT region. k, Structure-based sequence alignment (rendered with ESPript3⁷⁰) for human TPP1 (red) and Tetrahymena p50 (grey). Residues located on the interface are highlighted in yellow. Residues comprising TPP1 NOB and TEL patch are indicated on the top of alignment.