Extended Data Fig. 3: Colocalization of the rixosome with Polycomb bodies.
From: Rixosomal RNA degradation contributes to silencing of Polycomb target genes
a, b, Immunoblot validation of siRNA-mediated WDR18 (a) and EZH2 (b) knockdowns. c, Validation of siRNA knockdowns (48 h after transfection) of rixosome subunits, RING1B, and EZH2. d, Immunofluorescence colocalization of rixosome subunits MDN1 with EZH2-stained Polycomb bodies in cells treated with the indicated siRNA. DNA was stained with DAPI (blue). Scale bar, 5 μm. e, Quantification of MDN1 and EZH2 fluorescence intensity in d. p values are from two-sided student’s t-tests. Data are presented as mean values +/− SEM. f, Immunofluorescence of MDN1 (green), the nucleolar NPM1 protein (purple), and EZH2-stained foci (yellow). DNA was stained with DAPI (blue). g, Quantification of overlap between MDN1 foci and EZH2 or NPM1 per nucleus in the wild type cells. p values are from two-sided student’s t-tests. Data are presented as mean values +/− SEM.
