Extended Data Fig. 8: DNA methylation (measured by RRBS) status of human PSC converted by 4CL and e4CL.
From: Rolling back human pluripotent stem cells to an eight-cell embryo-like stage
a. Violin plot showing global CpG methylation levels measured by RRBS of human PSC cultured in primed conditions (n = 2 technical replicates), 4CL (day 12) (n = 2 technical replicates), 5iLAF35 (n = 2 technical replicates), t2iLGö-NK236 (n = 3 technical replicates), NHSM29 (n = 3 technical replicates), stepwise e4CL (day 5) (n = 2 technical replicates) and direct e4CL (day 7) (n = 3 technical replicates), human 8C-embryo (n = 3 biological replicates with 2 technical replicates each) and ICM32 (n = 2 technical replicates). All reference datasets used in this study are summarized in Supplementary Table 8. Our dataset was generated using H9 ESC. For boxplots, the blue central line is the median, the boxes indicate the upper and lower quartiles. b. Heatmap showing CpG methylation levels at a panel of imprinting control regions of human PSC cultured in primed conditions, 4CL (day 12), 5iLAF17, tt2iLGö37, NHSM29 and stepwise e4CL (day 5), ICM and postimplantation embryo32. c and d. Genome browser tracks showing CpG methylation levels at the indicated naïve pluripotency (blue) (c) and totipotency (red) loci (d) of PSC cultured in primed conditions, 4CL (day 12), 5iLAF, t2iLGö, NHSM, stepwise e4CL (day 5) and direct e4CL (day 7), human 8C-embryo and ICM. Each bar represents a single CpG and the height indicates the percentage of methylation.
