Extended Data Fig. 9: DNA methylation (measured by WGBS) status of primed human PSC converted by 4CL and sorted 8CLC.
From: Rolling back human pluripotent stem cells to an eight-cell embryo-like stage
a. Bar plot comparing global CpG methylation levels in the human 8C-embryo, ICM and postimplantation embryo32, and primed ESC, 4CL-day 12 naïve ESC and sorted 8CLC from e4CL-day 5. All reference datasets used in this study are summarized in Supplementary Table 8. Our dataset was generated using H9 ESC. b. Comparative DNA methylation level at the TSS, gene body and TES in primed ESC, 4CL-day 12 naïve ESC and sorted 8CLC. c. Comparative DNA methylation level in different genomic regions in ESC cultured in primed, 4CL-day 12 naïve ESC and sorted 8CLC. d. Genome browser tracks visualization of DNA methylation levels measured by WGBS at the indicated naïve pluripotency (blue) and totipotency (red) loci of primed ESC, 4CL-day 12 naïve ESC and sorted 8CLC. Each bar represents a single CpG and the height indicates the percentage of methylation. e. Comparative DNA methylation level at human early embryo-enriched TE (MLT2A1, MLT2A2 and LRT12C) in primed ESC, 4CL-day 12 naïve ESC and sorted 8CLC. f. Genome-wide comparative DNA methylation profiles in human 8C-embryo, ICM, postimplantation embryo, primed ESC, 4CL-day 12 naïve ESC, sorted 8CLC from e4CL-D5, 5iLAF35 and NHSM29. Data from different biological replicates were pooled and binned into consecutive 10-kb tiles. Only tiles covered by all the datasets were shown.
