Extended Data Fig. 1: Infection of human respiratory cells and distribution of SARS2-N501YMA30 antigen in sinonasal cavity and brain.
From: Eicosanoid signalling blockade protects middle-aged mice from severe COVID-19
a, Quantification of genomic RNA (gRNA), subgenomic RNA (sgRNA) and virus titers in Calu-3 cells at the indicated times after infection with 0.01 MOI of the indicated viruses. Data in a are geometric mean ± geometric SD and are representative of two independent experiments. b, Sinonasal cavity from young BALB/c mice infected with 5000 PFU of SARS2-N501YMA30, H&E stain (top panels). Regions of respiratory epithelium and olfactory epithelium exhibited uncommon regional scattered (bottom-left panel) to localized SARS-CoV-2 nucleocapsid immunostaining, (bottom panels). Scale bar 90 µm. OE: olfactory epithelium. c, Summary scores of nucleocapsid staining, as described in Methods (n = 8 at 2, 4 dpi; n = 6 at 6 dpi). Data in c are mean ± s.e.m. d, Brains from uninfected or infected young BALB/c mice at 6 dpi lacked overt lesions, H&E stain. Scale bar 45 µm. e, Brains from uninfected mice or young BALB/c mice infected with 5000 PFU at 2,4, and 6 dpi revealed no SARS-CoV-2 nucleocapsid immunostaining. Scale bar 460 µm. Data were pooled from two independent experiments. Data in b,d are representative of two independent experiments with similar results
