Extended Data Fig. 4: Lymphopenia in SARS2-N501YMA30-infected middle-aged mice.
From: Eicosanoid signalling blockade protects middle-aged mice from severe COVID-19
Numbers of immune cells in the blood of young and middle-aged C57BL/6 mice at 3 and 6 days after infection with 5000 PFU of SARS2-N501YMA30 (n = 9 for uninfected controls in both groups; n = 14 for middle-aged mice at 3 and 6 dpi; n = 12 for young mice at 3 and 6 dpi). a. CD45+ cells; b. PMN: Polymorphonuclear cells. c. B and T cells. P values determined by two-tailed Student’s t test. P values indicated are determined from the corresponding time point compared to uninfected control of the same group. P = 0.0483 (6 dpi vs uninfected, young C57BL/6; PMN); P = 0.0117 (3 dpi vs uninfected, middle-aged C57BL/6, PMN); P = 0.0016 (6 dpi vs uninfected, middle-aged C57BL/6, PMN); P = 0.0264 (3 dpi vs uninfected, young C57BL/6; B cells); P = 0.0001 (3 dpi vs uninfected, 6 dpi vs uninfected middle-aged C57BL/6, B cells); P = 0.0109 (6 dpi vs uninfected, young C57BL/6; Total T cells); P = 0.0001 (3 dpi vs uninfected, middle-aged C57BL/6, Total T cells); P < 0.0001 (6 dpi vs uninfected, middle-aged C57BL/6, Total T cells); P = 0.0072 (6 dpi vs uninfected, young C57BL/6; CD4+ T cells); P < 0.0001 (3 dpi vs uninfected, 6 dpi vs uninfected, middle-aged C57BL/6, CD4+ T cells); P = 0.0065 (6 dpi vs uninfected, young C57BL/6; CD8+ T cells); P = 0.0001 (3 dpi vs uninfected, middle-aged C57BL/6, CD8+ T cells); P < 0.0001 (6 dpi vs uninfected, middle-aged C57BL/6, CD8+ T cells). *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001. Data in a are mean ± s.e.m. Data are pooled from two independent experiments
