Extended Data Fig. 3: Recognition of cancer cell antigens by NK1.1⁺ αβILTCK-TCRs.

a, A representative flow plot showing the gating strategy used to isolate NK1.1⁺ αβILTCKs and PD-1⁺ T cells (TCs) from PyMT mice. NK1.1⁺ αβILTCKs are identified as CD45⁺TCRβ⁺CD8α⁺PD-1^–NK1.1⁺ whereas PD-1⁺ TCs are defined as CD45⁺TCRβ⁺CD8α⁺PD-1⁺NK1.1^–. b, A histogram showing the distribution of CDR3 lengths of TCRα and TCRβ pairs isolated from αβILTCks and PD-1⁺ TCs. Data are pooled from two independent experiments. c, Flow cytometric analysis showing the frequency of GFP⁺ cells among CD8⁺ and CD8^– reporter cell line expressing indicated TCRs 24 h after co-culturing with primary PyMT cancer cells or splenocytes pulsed with the SIINFEKL peptide (OVA_257-264). Data are representative of three independent experiments. d, Frequency of GFP⁺ cells among CD8^– reporter cell line expressing indicated NK1.1⁺ αβILTCK-derived TCRs 24 h after co-culturing with primary PyMT cancer cells. n = 3 for each TCR. All statistical data are shown as mean ± S.D (biologically independent mice in a–c and independent samples in d).

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