Extended Data Fig. 9: Length adjustment reduces upon perturbing molecules involved in surface tension.
From: Left–right symmetry of zebrafish embryos requires somite surface tension
(A) Comparison of AP length LAP under different perturbations. (B) Injected RGD-FITC localizes to notochord and somite boundaries. Scale bar, 50 μm. (C) Injection of 1.5 ng each of fn1a and fn1b MO or 4 ng of itgα5 MO results in disintegration of anterior somites by the 10th somite stage (zoomed in inset). Injection of 2 ng of cdh2 MO results in defective formation of neuroectoderm and tail. Lesser amounts of each of these MOs as indicated in (A) were used to analyse length adjustments. Scale bar, 100 μm. (D) Estimated relaxation timescale τ for wild type anterior somites (black) and the different perturbations. Error bar, fit uncertainty. Further details on statistics and reproducibility can be found in the Methods section.
