Extended Data Fig. 2: Nuclear volumes of hair cells in the inner or outer compartment of controls and in various Tbx2 conditional KOs.
From: Tbx2 is a master regulator of inner versus outer hair cell differentiation
Blue dots represent nuclear volumes of hair cells from the inner compartment (I in the column labels), whether IHCs (controls) or ic-HCs (mutants). Red dots represent nuclear volumes of hair cells from the outer compartment (OHCs; O in the column labels). Sample size (n) is indicated in each column. The time of tamoxifen administration to Fgf8CreER; Tbx2F/F pups is indicated in the column labels as TAM@P0 to P9. (a,b) While in controls the nuclei of IHCs are statistically larger than those of OHCs, in the mutants (all Tbx2 cKOs as well as the double cKO of Tbx2 and Insm1) the nuclei of the inner compartment hair cells (ic-HCs) are statistically smaller than those of control IHCs (b) and indistinguishable from those of OHCs (a). Error bars indicate SD and column tops the averages. Statistical comparisons were performed by one-way ANOVA with a Bonferroni post test. **** indicates P<0.0001, while ns (not significant) indicates P>0.05 (for panel a, from left to right, P=0.0792 for Atoh1CreTbx2F/F; P>0.9999 for Gfi1CreTbx2F/F; P=0.1285 for Atoh1CreGfi1CreTbx2F/F; P>0.9999 for Fgf8CreERTbx2F/F + Tamoxifen at P0, P7 and P9)
