Extended Data Fig. 3: Non-linear capacitance parameters evince electromotility of ic-OHCs.
From: Tbx2 is a master regulator of inner versus outer hair cell differentiation
(a,a’) Hair cells in the position of IHCs (ic-OHCs) that lacked TBX2 (from Fgf8CreER; Tbx2F/F; R26LSL-tdTomato/+ treated with tamoxifen at birth) were identified by their red fluorescence (tdTomato+; a’), whereas control IHCs and OHCs were obtained from equally treated littermates that lacked Fgf8CreER (Tbx2F/F; R26LSL-tdTomato/+), so that their IHCs did not transdifferentiate into ic-OHCs, expressing IHC markers like Calb2 and not tdTomato (a). These control IHC and OHC were distinguished as before15. Images shown are examples of results obtained in at least three separate tissue samples (n=3 biologically independent samples). (b) Summary of the non-linear capacitance (NLC) parameters of ic-OHCs lacking TBX2 and control OHCs (including those examined in Fig. 1s, t). Like control OHCs (n=9), all examined ic-OHCs (n=10) were electromotile. Samples of each cell type were obtained from ≥ 3 separate animals. Horizontal bars indicate the means and the standard deviations. Statistical comparisons were performed by unpaired t-tests (two-tailed). “ns” indicates not significant (p > 0.05)
