Extended Data Fig. 10: Detection of TMEM106B peptides by mass spectrometry.
From: Amyloid fibrils in FTLD-TDP are composed of TMEM106B and not TDP-43
a, Sequence map of TMEM106B. Sequences in red indicate unique peptides detected by LC-MS/MS from excised gel bands. b, Fragmentation spectra (MS/MS) of detected peptides from TMEM106B. Detected fragment ions (b, y, and immonium ions) are labelled accordingly. The peptide modifications methionine oxidation (OX) and cysteine carbamidomethylation (CA) were observed. SDS-PAGE gel of sarkosyl-insoluble fraction of donor 1 shown as an insert. Box indicates the gel region, which corresponds to the ~35kDa band from the TMEM106B western blot (Fig. 1b), excised for LC-MS/MS analyses.
