Extended Data Fig. 1: Comparison of FTLD-TDP and non-FTLD-TDP donors.
From: Amyloid fibrils in FTLD-TDP are composed of TMEM106B and not TDP-43
a, Age distribution of FTLD-TDP donors (F, n = 40) and non-FTLD-TDP controls (N, n = 8). Donors with and without fibrils detected under negative stain EM are coloured black and blue, respectively. P-value of 0.53 (n.s., not significant) from an unpaired, two-tailed t-test suggests that the presence of fibrils is disease-dependent but not age-dependent. b, Western blots of sarkosyl-insoluble fractions from FTLD-TDP (F) and non-FTLD-TDP donors (N) probed by TMEM106B antibody. The ~35 kDa TMEM106B-positive band was found in none of the non-FTLD-TDP donors (N5 shown in Fig. 1b as donor 5) and all of the FTLD-TDP donors except F35, F3, and F27. P-value of less than 0.0001 was obtained from an unpaired, two-tailed t-test comparing presence of the ~35kDa band (value of “1” for present, “0” for absent) in non-FTLD-TDP donors (0 out of 8, n = 8) and FTLD-TDP donors (22 out of 25, n = 25). Fibrils were not observed in F3 and F27 by negative stain EM, consistent with the western blot. Fibrils were observed in F35, which suggests that western blot may not always be accurate in detecting TMEM106B aggregation. Western blot membranes were prepared in parallel and exposed with equal time. The original, uncropped blots are shown in Supplementary Fig. 3.
