Extended Data Fig. 2: Fibril screen of FTLD-TDP and non-FTLD-TDP donors by negative stain EM.
From: Amyloid fibrils in FTLD-TDP are composed of TMEM106B and not TDP-43
Negative stain EM images of sarkosyl-insoluble fractions from all donors. Scale bar 200 nm. Donors 1–5 (F26, F36, F17, F40 and N5, respectively) are also shown in Fig. 1a. Fibrils with similar morphologies were found in all FTLD-TDP donors except F3 and F27. No fibrils were found in any non-FTLD-TDP donors. P-value of less than 0.0001 was obtained from an unpaired, two-tailed t-test comparing fibrils detected by EM (value of 1 for present, 0 for absent) in FTLD-TDP donors (38 out of 40, n = 40) and non-FTLD-TDP donors (0 out of 8, n = 8).
