Extended Data Fig. 6: Aldoc+ Purkinje neurons show no obvious transcriptomic plasticity upon exposure to motor activity and learning.

a, ElbowPlot showing the ordered standard deviations of principal components (PCs) for identification of significant dimension that begins to plateau. Dim 8 was chosen at the standard deviations of 2. b, 19,424 Aldoc+ Purkinje nuclei isolated from the whole cerebellum or anterior vermis lobules under control, activity, motor learning or associative learning conditions were grouped into 4 clusters based on marker gene expression with cluster resolution of 0.2. c, 19,424 Aldoc+ Purkinje nuclei isolated from the whole cerebellum or anterior vermis lobules under control, activity, motor learning or associative learning conditions were grouped into 12 clusters based on marker gene expression with cluster resolution of 1.0. d, e, UMAP plot showing the grouped cluster landscape of Aldoc+ nuclei of different training paradigms under baseline, activity, learning or learned conditions. f, Bar plot showing the percentages of all 12 clusters with cluster resolution of 1.0 under different conditions described in c. g, Heat map showing the representative marker genes of each cluster (resolution of 0.5) of Aldoc+ Purkinje neurons isolated from the whole cerebellum or anterior vermis lobules. Expression values are presented as normalized Z-scores. h, Fos expression in the clusters of Plcb4+ and Aldoc+ Purkinje neurons at baseline or learning/learned condition.