Extended Data Fig. 2: Aldoc+ and Plcb4+ Purkinje neurons show distinct molecular and cellular features.
From: Transcriptomic mapping uncovers Purkinje neuron plasticity driving learning
a, 20,814 single nuclei of Purkinje neurons were subjected to principal component analysis. Heat map showed that the first principal component (60.9%) clustered Purkinje neurons into Plcb4+ and Aldoc+ populations. b, UMAP plot showing grouped Purkinje nuclei from the whole cerebellum or anterior vermis lobules into two primary categories, Aldoc+ and Plcb4+ Purkinje neurons. c, Nuclei of two biological replicates and sequencing batches from the whole cerebellum or anterior vermis lobules showed similar cell type clustering. d, Bar plot showing the percentages of Aldoc+ and Plcb4+ Purkinje neurons in the whole cerebellum and anterior vermis lobules. Data are presented as mean ± s.e.m. n = 2 in biologically independent snRNA-seq. 5870 Aldoc+ and 5675 Plcb4+ neurons in whole cerebellum; 3243 Aldoc+ and 6026 Plcb4+ neurons in anterior vermis lobules. e, The coronal sections through the anterior (up panel) or posterior (lower panel) cerebellar lobules were subjected to ISH analyses using fluorescence-labeled Aldoc (red) or Plcb4 (green) RNA probes. Scale bar, 800 μm. Similar results were found in 3 animals for representative data. f, Scatter plot depicting the expressions of DEGs in Plcb4+ and Aldoc+ Purkinje neurons in the anterior vermis lobules. Blue dots showing representative genes highly expressed in Plcb4+, while purple ones were those in Aldoc+ Purkinje neurons. g, Heat map showing the representative functional genes specifically expressed in Plcb4+ or Alodc+ Purkinje neurons isolated from the whole cerebellum or anterior vermis lobules. Expression values are presented as normalized Z-scores.
