Extended Data Fig. 3: Biochemical fractionation and mass spectrometry identification of phage T4 gene 57B as Acb1.
From: Phage anti-CBASS and anti-Pycsar nucleases subvert bacterial immunity
a, Schematic depicting strategies of biochemical fractionation to enrich 3′3′-cGAMP cleavage activity from crude T4 lysate. Mass spectrometry of active fractions revealed 37 candidate T4 proteins. b, Agarose gel analysis of 34 successfully PCR-amplified candidate T4 genes to be screened for 3′3′-cGAMP cleavage activity by in vitro translation. Data are representative of 2 independent experiments. For gel source data, see Supplementary Figure 1. c, Translation products from b were tested for 3′3′-cGAMP cleavage activity by TLC. Data are representative of 2 independent experiments.
