Extended Data Fig. 3: Further characterization of tumour distributions.

(a) Cleared brain of the CKO model at different tumour stage. Ms19127 contained no detectable tumour; Ms18980 carried a small tumour in the glomerular layer of the OB; Ms19119 harboured obvious tumour in the glomerular layer; and Ms18905 carried obvious tumour in both the glomerular layer and the GRL of the OB. Stri, striatum. THA, thalamus. Arrows point to tumours in the OB. (b) WES and tumour phylogenies for CKO mice (n = 2 mice) at the moribund stage. Three specimens were analysed in each mouse brain as indicated: N (normal brain tissue), T1 (tumour tissue in the OB) and T2 (tumour tissue in the cerebrum away from the OB). The phylogenetic trees illustrate the evolutionary relationship among the three specimens in each brain. For each branch, the number and the gene names of non-synonymous SNVs that were attributed to the branch are shown. Red signal indicates tdT. (c) Representative image of the coronal section from one mouse OB. Different layers within the OB are visualized with corresponding markers as indicated. Of note, M/T cells were labelled by the Igf1-EGFP transgene (see also Fig. 3), the GAD2 expression was visualized by using Gad2-Cre crossing with the reporter line Rosa26^LSL-tdTomato (Ai9). Therefore, the final genotype of this mouse is Igf1-EGFP^+/−; Gad2-Cre^+/−; Rosa26^{CAG-LSL-TdTomato +/−}. (d) Quantification of tumour incidence of the CKO model within the glomerular layer. (e) Normalized density of FOS⁺ cells in the glomerular layer (n = 3 mice). Scale bar: (c), 500 μm.