Extended Data Fig. 9: Fgf8 induces OPC proliferation and Fgf17 induces SRF reporter activation mediated by actin dynamics and Fgfr3.
From: Young CSF restores oligodendrogenesis and memory in aged mice via Fgf17
a, Dose-dependent activation of SRE-GFP reporter by increasing concentrations of Fgf8 and representative images of the experiment at 15.5 h. Scale bar, 400 μm. (n = 3; similar control as in Fig. 4c; one-way ANOVA followed by Sidak’s post-hoc test; mean ± s.e.m.). b, Percentage of BRDU+/DAPI primary rat OPCs treated with 10, 20, 40 ng/ml Fgf8. (n = 4; one-way ANOVA followed by Tukey’s post-hoc test; mean ± s.e.m.). c, Quantification of OPC proliferating cells (Pdgfrα+EDU+ / Pdgfrα+ cells) in the CA1 region of the hippocampus of 20-month-old mice following a week of aCSF or Fgf8 infusion. (aCSF n = 8 similar control as in Fig. 4l, Fgf8 n = 4; two-sided t-test; mean ± s.e.m.). d, SRE-GFP activation with 200 ng/ml Fgf17 following 30 min pre-treatment with Jasplakinolide (Jasp, 125 or 250 nM) or Latrunculin A (LatA, 250 or 500 nM). (n = 3; Two-way ANOVA with Tukey’s multiple comparisons test; mean ± s.e.m.). e, SRE-GFP activation with 200 ng/ml Fgf17 following 30 min pre-treatment with blocking antibodies for FgfR1, FgfR2, FgfR3 (all 50 μg/ml) or FgfR3 alone (n = 3; One-way ANOVA with Sidak’s multiple comparisons test; mean ± s.e.m.). f, Example of Pdgfrα+ Fgfr3+ cells in the hippocampus of young mice. (n = 3). Scale bar, 5 μm.
