Extended Data Fig. 1: Bulk RNAseq, infusion site details and overall overview of proliferating cells.
From: Young CSF restores oligodendrogenesis and memory in aged mice via Fgf17
a, Relative proportions of cell types as predicted by deconvolution analysis of bulk RNAseq of aged mice infused with aCSF or YM-CSF (aCSF n = 8, YM-CSF n = 7). b, Predicted number of DEGs per cell type by deconvolution analysis of bulk RNAseq of aged mice infused with aCSF or YM-CSF (aCSF n = 8, YM-CSF n = 7). c, Effect size of the subset of oligodendrocyte genes in Fig. 1d 16 h following acute injection of YM-CSF or aged mouse CSF (AM-CSF) calculated over aCSF as control (n = 4; Wilcoxon rank sum test). d, Location of infusion site. Image source: Allen Institute, Mouse brain atlas (coronal). e, Location of analysis site. Image source: Allen Institute, Mouse brain atlas (coronal). f, Hippocampal slice of 10-month-old mice given an EdU pulse prior to surgery showing low baseline proliferation, and three pulses of BrdU at day 5 and 6 of infusion showing an overall increase in proliferating cells following YM-CSF infusion (n = 4 per group; repeated measures two-way ANOVA followed by Sidak’s post-hoc test; Means ± SEM). g, Representative images of EdU (red) and BrdU (green) cells in mice with no surgery or infused with aCSF or YM-CSF. Scale bar, 500 μm. h, RNAscope of Pdgfrα+EdU+ cells in hippocampus of 2-month-old (young) and 19-month-old (aged) mice (n = 3; two-sided t-test; mean ± s.e.m.). i, Representative images of analysis in panel h. Arrows pointing to Pdgfrα+EdU+ cells. Scale bar, 100 μm.
