Extended Data Fig. 3: Identification of TR proteins in reactive astrocytes by immunohistochemistry.

For immunohistochemical (IHC) detection of TR protein we focused on previously unpublished, TREA-predicted astrocyte reactivity TRs for which sensitive antibodies were commercially available whose specificity was supported by western blots. a. Newly identified TRs co-localized by IHC in reactive astrocytes in thoracic spinal cord (T9-T-10) after LPS treatment. TRs are show in alphabetical sequence. b. Summary of all (newly identified and previously published) astrocyte reactivity TRs after LPS treatment identified here by at least two experimental procedures, either prediction from DEGs by TREA, or prediction based on significant change in motif access determined by ATAC-seq or by IHC or by all three. c. Newly identified TRs co-localized by IHC in reactive astrocytes in thoracic spinal cord (T9-T-10) after SCI (continued in Extended Data Figs. 4, 5). TRs are show in alphabetical sequence. Each immunohistochemical evaluation was repeated at least three times with similar results.