Extended Data Fig. 9: Comparisons of genomic DNA and RNA expression patterns in A. thaliana accessions Col-0 and Sha.
From: An oxygen-sensing mechanism for angiosperm adaptation to altitude
a,b Introduction of the prt6-1 mutation (by introgression from low altitude accession Col-0) or C2A-RAP2.3 (by transformation) into A. thaliana high altitude accession Sha enhances the expression of hypoxia-related genes. c, Expression of RNAs for PCO PRT6 N-degron pathway components in accessions Sha and Col-0. RNA was extracted using etiolated seedlings after 5 days growth at pO2 21.2 kPa (48 m a.s.l). d, Comparison of DNA sequence of the first coding exon of FLU in accessions Col-0 and Sha. DNA sequence information was obtained from Arabidopsis 1001 web site: http://signal.salk.edu/atg1001/2.0/gebrowser.php. Shown are ERFVII binding site (also known as EBP, GCC box) and related HRPE (Hypoxia-Responsive Promoter Element2)-like element in the first coding exon of A. thaliana FLU (initiating ATG highlighted). All experiments carried out using etiolated seedlings after 5 days growth. Means are plotted, error bars report SD. Significant differences denoted with letters for one-way ANOVA (p < 0.05). For each n = 3 biologically independent experiments.
