Fig. 2: Steady-state levels of Pchlide and FLU expression are determined by altitude. | Nature

Fig. 2: Steady-state levels of Pchlide and FLU expression are determined by altitude.

From: An oxygen-sensing mechanism for angiosperm adaptation to altitude

Fig. 2

a, Pchlide levels in A. thaliana accessions collected at different altitudes and geographic locations30, grown at a pO2 of 21.2 kPa. Grey dots show pO2 at the different altitudes from which the accessions were collected. Genomic groups are indicated in different colours. IP, Iberian Peninsula b, Pchlide levels in S. habrochaites grown at 48 m a.s.l. with 21% and 15% ambient O2. c, Effect of ambient O2 concentration on Pchlide levels in A. thaliana accessions grown at 48 m a.s.l. (R2 values from Supplementary Table 1b). d, e, Effect of ambient O2 concentration on amount of FLU mRNA in A. thaliana (d) and S. habrochaites (e) grown at 48 m a.s.l. f, Pchlide levels in cultivated domesticated C. quinoa accessions obtained from different altitudes grown at pO2 21.2 kPa. g, Comparison of Pchlide levels in A. thaliana accessions collected at different altitudes and grown at 48 m a.s.l. with pO2 = 21.2 kPa or 15% ambient O2, or at 2,479 m a.s.l.(pO2 = 15.7 kPa). h, FLU RNA accumulation in A. thaliana accessions collected at different altitudes and grown at 48 m a.s.l. (pO2 = 21.2 kPa) or at 2,479 m a.s.l. (pO2 = 15.7 kPa). All experiments were carried out using etiolated seedlings after 5 days growth. Data are mean ± s.d. Accessions used are listed in Supplementary Table 1a. n = 3 biologically independent experiments.

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