Fig. 3: Genetic mechanisms linking oxygen sensing to altitude adaptation.
From: An oxygen-sensing mechanism for angiosperm adaptation to altitude
a, Effect of ambient O2 on RNA accumulation of hypoxia-associated ADH1 in S. habrochaites and A. thaliana accessions. b, Pchlide levels in prt6-5, 35S:PCO2, prt6-5 35S:PCO2 and Col-0. c, d, Pchlide level and FLU, PORA and PORB transcript expression in prt6-1 mutants and transgenic plants expressing wild-type or Cys2Ala mutant Col-0 RAP2.3 or HRE2 (driven by their own promoters) in Sha and Col-0 genetic backgrounds. e, ChIP analysis of HRE2–HA occupancy at FLU −49:+84 and hypoxia-related genes in Col-0 and Sha seedlings grown with pO2 21.2 kPa or 15% ambient oxygen. f, Western blot analysis of HRE2–HA in Sha and Col-0 accessions grown at pO2 21.2 kPa. The experiment was repeated independently three times with similar results. BZ, bortezomib. g, A model for angiosperm adaptation to altitude through oxygen sensing. Wedges indicate decreasing pO2 with increasing altitude. Blocked arrows indicate repression. Arrow-crossed box is international standard symbol for a rheostat. The inactivation complex model is adapted from ref. 16. All experiments were carried out using etiolated seedlings after 5 days growth at 48 m a.s.l. Data are mean ± s.d.; one-way ANOVA. Significantly different groups are indicated by letters in c, d. n = 3 biologically independent experiments.
