Extended Data Fig. 1: Tetrapyrrole synthesis and Pchlide steady state levels in etiolated seedlings.
From: An oxygen-sensing mechanism for angiosperm adaptation to altitude
a, Schematic of tetrapyrrole synthesis showing points of oxygen requirement3. Blue, enzymes, black metabolites. Blocked arrows indicate repression. ALA, 5-aminolevulinic acid; Mg-Proto, Mg-protoporphyrin; GluTR, glutamyl-tRNA reductase; CHLM, MAGNESIUM-PROTOPORPHYRIN IX METHYLTRANSFERASE; HY1, HEME OXYGENASE1; POR, Light requiring PCHLIDE OXIDOREDUCTASE; FLU, FLOURESCENT IN BLUE LIGHT. Constituents of the GluTR inactivation complex are shown, diagram after16. b, Linearity of the measurement of Pchlide peak fluorescence at 636 nm, measured as arbitrary units (a.u.) per cotyledon per mm2 (to account for differing cotyledon sizes between accessions of the same species), using the Pchlide over-accumulating Col-0 mutant flu. c, Pchlide levels in Col-0 (wild type) and flu. d, Levels of Pchide in A. thaliana during 7 days of etiolated growth of Col-0 (wild-type) and flu. Experiments carried out using etiolated seedlings after 5 days growth at pO2 21.2 kPa (48 m a.s.l) unless otherwise stated. Means are plotted, error bars report SD. For each n = 3 biologically independent experiments.
