Extended Data Fig. 2: The influence of the PRT6 N-degron pathway and ERFVIIs on Pchlide levels.
From: An oxygen-sensing mechanism for angiosperm adaptation to altitude
a, Pchide steady state levels in A. thaliana during 7 days of etiolated growth of Col-0 (wild-type) and N-degron pathway mutant prt6-1 and erfVII combinations. b, Pchlide levels at pO2 21.2 kPa (48 m a.s.l) in A. thaliana N-degron pathway mutant prt6-1 and erfVII combinations and individual ERFVII mutants. c, Pchlide levels in prt6, flu and prt6 flu. d, Analysis of RNA expression of FLU and genes of tetrapyrrole synthesis in Col-0 (wild type) and erfVII and prt6 mutants. e, Transcript levels in Col-0 and erfVII at different ambient oxygen levels. f, Accumulation of proteins for POR and FLU in Col-0 (wild type) and PRT6 N-degron pathway mutants at pO2 21.2 kPa (48 m a.s.l), and in Col-0 and erfVII at different ambient oxygen levels, repeated independently three times with similar results. All experiments carried out using etiolated seedlings after 5 days growth at pO2 21.2 kPa (48 m a.s.l) unless otherwise stated. Means are plotted, error bars report SD. Significant differences denoted with letters for one-way ANOVA (p < 0.05). Coefficient of determination (R2) is given. For each n = 3 biologically independent experiments.
