Extended Data Fig. 3: ChIP analysis of HRE2 interaction with tetrapyrrole synthesis-related genes.
From: An oxygen-sensing mechanism for angiosperm adaptation to altitude
a, Conservation of ERFVII binding site (also known as EBP, GC box) and related HRPE (Hypoxia-Responsive Promoter Element38)-like element in the first coding exon of A. thaliana FLU and orthologues from selected angiosperms (initiating ATG highlighted). b, Schematic of the CHLM gene (repressed by ERFVIIs) showing ERFVII-binding sites and ChIP analysis of RAP2.3-3xHA and HRE2-3xHA occupancy of CHLM gene regions (including known positive and negative sequences29). c,d Conservation of ERFVII binding site in the first coding exons of CHLM and CHL27 respectively. e, ChIP analysis of HRE2-3xHA occupancy of gene regions of CHL27, PORA, PORB and HEMA1. f, Schematics of genes showing positions of ERFVII and ERFVII-like binding sites. White boxes untranslated and black boxes translated regions. All experiments carried out using etiolated seedlings after 5 days growth at pO2 21.2 kPa (48 m a.s.l). Means are plotted, error bars report SD. For each n = 3 biologically independent experiments.
