Extended Data Fig. 9: Specific expression of smed-glp in the male reproductive organ (penis papillae) and female reproductive organ (bursa canal).

Expression of the glp gene (in red color) and the stem cell marker smedwi-1 (in green color) in the dissected copulatory apparatus was visualized by Fluorescence in situ hybridization chain reaction. (A) White dashed lines outline penis papillae and bursa canal. Nuclei were stained by DAPI (in blue color). No glp expression in tissues surrounding penis papillae or bursa canal. Less than 1% of glp+ cells express the stem cell marker smedwi-1. (B) Cells expressing the two markers do exist in both bursa canal and penis papillae. This experiment was repeated 3 times independently with 6 sexually mature worms, producing similar results.