Extended Data Fig. 11: Steady-states levels of mitoribosomal subunits and assembly factors in GTPBP10 and MRM3 knockout cells.
From: Mechanism of mitoribosomal small subunit biogenesis and preinitiation
a, Steady-states levels of mitoribosomal subunits and assembly factors in GTPBP10 and MRM3 knockout cells. Steady-state levels of mitoribosomal proteins (uS16m, mS35, mS37, mL37), assembly and initiation factors (MRM3, RBFA, METTL15, IF3) in the WT HEK293T, MRM3 knockout, MRM3- rescue, GTPBP10 knockout and GTPBP10-rescue cells were analyzed by immunoblotting with corresponding antibodies. SDHA was used as a loading control. Representative gels from three independent biological experiments are shown. b, SILAC-based proteomic analysis of sucrose gradient fractions. Protein steady-state levels of SSU (yellow) and LSU (blue) components in MRM3KO cells are presented relative to WT HEK293T (n = 3 biologically independent experiments). Limma t-test was performed; the adjusted P-value for mS37 is 0.000521. For source data, see Supplementary Fig. 4.
