Fig. 1: A chimeric orthogonal IL-2 receptor reveals properties of IL-9R signalling in T cells.
From: Potentiating adoptive cell therapy using synthetic IL-9 receptors
a, Schematic of wild-type IL-2Rβ, orthogonal IL-2Rβ or γc family chimeric orthogonal receptor complexes (created with Biorender.com). b, Representative histogram and quantification of pSTAT signalling in chimeric-orthogonal-receptor-expressing (YFP+) or untransduced (UTD) T cells stimulated with MSA-IL-2 (100 nM) (unfilled colour) or MSA-oIL-2 (5 μM) (filled colour) for 20 min. Data are shown as mean fluorescence intensity (MFI). c, Dose–response curves of pSTAT signalling in YFP+ o2R (red) or o9R (blue) transduced T cells stimulated with MSA-oIL-2, MSA-IL-2 or IL-9 for 20 min. d–f, Surface marker levels of CD62L (c), Fas (CD95) (e) and Sca-1 (f) of chimeric-orthogonal-receptor-expressing T cells cultured with MSA-IL-2 (100 nM) or MSA-oIL-2 (5 μM) for two days. NS, not significant; *P < 0.05, ***P < 0.001, ****P < 0.0001 (ANOVA). g, Dose–response curves of YFP+ o2R or o9R cells that have undergone at least one division after four days of culture in MSA-oIL-2 or MSA-IL-2. Data are shown as the percentage divided. Data are mean ± s.e.m. with n = 3 biological replicates, unless stated otherwise.
